The HTRF human CXCL1 (GRO alpha) kit is designed for the quantification of human CXCL1 release in cell supernatant
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Also called GRO alpha, KC NAP-3 (neutrophil activating protein 3), or MGSA-alpha (melanoma growth stimulating activity alpha), CXCL1 is a member of CXC chemokine family. Mainly expressed by macrophages, CXCL1 attracts neutrophils and is involved in angiogenesis, inflammation, and tumorigenesis.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
Assay Points |
500
|
---|---|
Assay Technology |
HTRF
|
Brand |
HTRF
|
Product Group |
Kit
|
Quantity |
1
|
Shipping Conditions |
Shipped in Dry Ice
|
Therapeutic Area |
Oncology & Inflammation
|
Unit Size |
500 Assay Points
|
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
Revvity also worked with Myassays.com to help you in your data analysis.
Sample size | 16 µL |
---|---|
Final assay volume | 20 µL |
Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
LOD &LOQ (in Diluent) | 13 pg/mL &47 pg/mL |
Range | 47 4,000 pg/mL |
Time to result | 2h at 4°C |
Species | Human only |
Intra-assay (n=24)
Sample | Mean [CXCL1] (pg/mL) | CV |
---|---|---|
1 | 150 | 8% |
2 | 1500 | 6% |
3 | 3000 | 9% |
Mean CV | 8% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
Sample | [CXCL1] (pg/mL) | Mean (delta R) | CV |
---|---|---|---|
1 | 212 | 350 | 13% |
2 | 686 | 934 | 2% |
3 | 2222 | 1904 | 1% |
Mean CV | 5% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
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