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HTRF Human CCL3 (MIP1α) Detection Kit, 500 Assay Points
The HTRF human CCL3 (MIP1 alpha) kit is designed for the quantification of human CCL3 release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
Also called MIP-1 alpha (macrophage inflammatory protein 1 alpha), CCL3 is a member of MIP (macrophage inflammatory protein) family, which also includes CCL4 (MIP1 beta). This chemokine is secreted by monocytes, macrophages, T cells, and endolial cells. CCL3 plays an important role in activation of inflammatory response by attracting monocyte-lineae cells and lymphocytes to inflammation sites. CCL3 has been reported as a stem cell inhibitory factor.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
Specifications
| Assay Points |
500
|
|---|---|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Product Group |
Kit
|
| Quantity |
1
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Therapeutic Area |
Metabolism/Diabetes
Oncology & Inflammation
|
| Unit Size |
500 Assay Points
|
Video gallery
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
Revvity also worked with Myassays.com to help you in your data analysis.
Assay details
Technical specifications of human CCL3 kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol |
| LOD &LOQ (in diluent) | 28 pg/mL &58 pg/mL |
| Range | 58 4,400 pg/mL |
| Time to result | ON @ RT |
| Calibration | NIBSC (92/518) value (IU/mL) = 0.00005 x HTRF hCCL3 value (pg/mL) |
| Species | Human only |
Analytical performance
Intra and Inter assay
intra assay (n=24)
| Sample | Mean [CCL3] (pg/ml) | CV |
|---|---|---|
| 1 | 133 | 9% |
| 2 | 667 | 4% |
| 3 | 3450 | 4% |
| Mean CV | 6% |
inter assay (n=4)
| Sample | [CCL3] (pg/ml) | Mean (delta ratio) | CV |
|---|---|---|---|
| 1 | 178 | 298 | 8% |
| 2 | 642 | 1138 | 3% |
| 3 | 2316 | 4682 | 4% |
| Mean CV | 5% |
Assay validation
CCL3 secretion in PBMC stimulated with LPS, IL1ß and TNFa
A dose response of LPS was performed on PBMC plated at 100,000 cells / well / 100 µL in a 96 well plate. Supernatants were diluted at 1/8th and 16µl of diluted samples were transferred into a Revvity 96-well low volume white plate to be analyzed by the Human CCL3 kit.
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SDS, COAs, Manuals and more
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Safety data sheet
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Certificate of analysis
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Lot number02ELot dateJuly 28, 2024
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IFUs, Manuals & more
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Resource typeManualLanguageEnglishCountry-
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