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HTRF Human ApoE Detection Kit, 500 Assay Points

The ApoE kit is designed for the simple quantification of ApoE in cell-based formats.

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Feature	Specification
Application	Protein Quantification
Sample Volume	10 µL

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The ApoE kit is designed for the simple quantification of ApoE in cell-based formats.

View product information

Product Variants

Part #:

64APOEPEG

List Price

USD 1,429.62

Your online price:

Part #:

64APOEPEH

List Price

USD 14,327.50

Your online price:

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

Product information

Overview

Apolipoproteins are proteins that bind to lipids to form lipoproteins, whose main function is to transport lipids. Apolipoproteins are key in maintaining the structural integrity and solubility of lipoproteins. ApoE is a fat-binding protein that is part of the chylomicron and Intermediate-Density Lipoprotein (IDLs). It is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. A correlation between ApoE and neurodegenerative conditions such as multiple sclerosis and Alzheimer's disease has been found.

Specifications

Other Specifications

Application	Protein Quantification
Automation Compatible	Yes
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	10 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Biomarkers
Target Species	Human
Technology	TR-FRET
Therapeutic Area	Cardiovascular Metabolism/Diabetes NASH/Fibrosis Neuroscience
Unit Size	500 assay points

Video gallery

How it works

Assay principle

The Apolipoprotein E assay is a sandwich immunoassay involving two monoclonal antibodies, one labelled with Eu-Cryptate (Donor) and the second with d2 (Acceptor). The intensity of the signal is proportional to the concentration of apolipoprotein present in the sample.

Assay protocol

The ApoE assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. This protocol requires a single 3-hour incubation period at RT.

Assay details

Apolipoprotein E standard curve

10 µL of recombinant protein in diluent #5 were dispensed to a low volume white microplate before the addition of 10 µL of the premixed HTRF anti ApoE detection reagents. The HTRF signal was recorded after 2h incubation at room temperature.