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Tag-lite HaloTag-Green Labeling Reagent, 5 nmoles

SNAP-Tag, Clip-Tag, and HALO-Tag are protein labeling systems enabling the covalent attachement of fluorophores onto proteins of interest. HaloTag-Green enables HALO-tagged proteins to become labeled with a green fluorophore.

Feature Specification
Application Receptor-Ligand Binding

SNAP-Tag, Clip-Tag, and HALO-Tag are protein labeling systems enabling the covalent attachement of fluorophores onto proteins of interest. HaloTag-Green enables HALO-tagged proteins to become labeled with a green fluorophore.

Product Variant
Unit Size: 5 nmol
Part #:
SHALOGRNE
List Price
USD 435.04
For research use only. Not for use in diagnostic procedures.

Overview

Over the past few years, SNAP-Tag, CLIP-Tag, and Halo-Tag technologies combined with TR-FRET have paved the road to the development of many non-radioactive, no-wash binding assays. The method is based on transfecting cells using plasmids encoding a SNAP-Tag (or CLIP-Tag, or Halot-Tag) and subsequently labeling them with acceptors or donors. HaloTag-Green allows the labeling of Halo-Tagged proteins with a green fluorophore.

Specifications

Application
Receptor-Ligand Binding
Brand
Tag-lite
Detection Modality
HTRF
Product Group
Fluorescent Reagent
Shipping Conditions
Shipped in Dry Ice
Target Class
GPCR
Technology
TR-FRET
Therapeutic Area
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
Unit Size
5 nmol

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Technical Note
A simple method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay

G-protein coupled receptors (GPCRs) are crucial transmembrane proteins involved in cellular signal transduction.

This technical...

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