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This cell-based assay format enables the detection of the H3K36Me2 motif, as a result of histone H3 methylation by EZH2 enzyme among others.
This cellular assay has been developed with optimized reagents and protocols for the direct detection of endogenous levels of H3K36Me2. This HTRF® format uses a very specific antibody pair combined with a highly efficient nucleus extraction which enables the assay to be used with a variety of cell backgrounds, and for the study of important methyltransferases and demethylases targets, such as JMJD2A. The H3K36Me2 assay can be used for adherent or suspension cells, primary or secondary screening and inhibitor studies.
|Assay Target Type
Oncology & Inflammation