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HTRF Tag-Lite GIP Receptor Red Agonist Ligand, 5,000 Assay Points

This GIP receptor red agonist is a GIP derivative labeled with a red emitting HTRF fluorescent probe.

For research use only. Not for use in diagnostic procedures.
Part number: L0018RED
List price: USD 2,254.00
Your price:
USD 2,254.00
USD 2,254.00 /each

Overview

Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.

This GIP receptor red agonist is a GIP derivative labeled with a red emitting HTRF fluorescent probe.

This ligand is best suited for use in the Glucagon GIPR receptor binding assay.

Specifications

Assay Points
5000
Assay Target Type
Fluorescent reagent
Assay Technology
HTRF
Brand
Tag-Lite
Quantity
1
Therapeutic Area
Metabolism/Diabetes
NASH/Fibrosis
Unit Size
5,000 Assay Points

Video gallery

How it works

Assay principle

Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

gpcr-how-it-works-assay-principle
Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

gpcr-how-it-works-saturation-binding
how-it-works-glucagon-gip-receptor
Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.

gpcr-how-it-works-competitive-binding-ki-l0018red
how-it-works-glucagon-gip-receptor-red-ago

 

Resources

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Flyer
Download your tag-lite brochure

Tag-lite™ is a cell-based, no-wash alternative to traditional radioisotope assays for studying GPCR-ligand interaction.

Adenosine...

Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

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