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HTRF Europium NH2 Labeling Reagent, 1 mg
HTRF Europium NH2 Labeling Reagent, 1 mg
Revvity Vial from a Kit, Standard, or Control lysate
Europium cryptate-NH2 labeling reagent enables Europium cryptate labeling of biomolecules.
| Feature | Specification |
|---|---|
| Application | Labeling |
Europium cryptate-NH2 labeling reagent enables Europium cryptate labeling of biomolecules.
Product variants
Unit Size: 100 µg
Part #:
65EU2ABA
List price
USD 3,395.00
Your online price:
Unit Size: 1 mg
Part #:
65EU2ABB
List price
USD 22,635.00
Your online price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Europium NH2 Labeling Reagent, 1 mg
Revvity Vial from a Kit, Standard, or Control lysate
HTRF Europium NH2 Labeling Reagent, 1 mg
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Product information
Overview
Europium cryptate-NH2, is a do-it-yourself solution for labeling peptides, oligonucleotides or proteins with Europium cryptate.This reagent is ideal for experienced users familiar with labeling and chromatography. Used in combination with bi-functional reagents such as carbodiimides and SMCC/SPDP. Maleimide group generated by SMCC activation of the Europium cryptate-NH2 reacts with the thiol generated by SPDP/DTT treatment on the biomolecules to label.
HTRF assays offer many advantages over other technologies:
- Homogeneous add-and-read format
- No wash steps
- Low background
- Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
- Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay protocol
As shown here, preactivation of both the Europium cryptate and the biomolecule may be required. Recovery of labeled material can be achieved using conventional chromatographic methods, such as gel filtration or HPLC. The purification devices required for recovering labeled material are not delivered with these reagents.
Specifications
| Application |
Labeling
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Fluorescent Reagent
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Technology |
TR-FRET
|
| Unit Size |
1 mg
|
Video gallery
HTRF Europium NH2 Labeling Reagent, 1 mg
HTRF Europium NH2 Labeling Reagent, 1 mg
Resources
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Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
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