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HTRF Tag-Lite Dopamine D2 Receptor Red Antagonist Ligand, 5,000 Assay Points

This Dopamine D2 receptor red antagonist is a spiperone derivative labeled with a red emitting HTRF fluorescent probe, to be used in the Tag-lite system.

For research use only. Not for use in diagnostic procedures.
Part Number: L0002RED
Part Number: L0002RED
  • Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function. This Dopamine D2 receptor red antagonist is a spiperone derivative labeled with a red emitting HTRF fluorescent probe.

    This ligand is best suited for use in the Dopamine D2 and Dopamine D3a receptor binding assays.

  • Assay Points
    5000
    Assay Target Type
    Fluorescent reagent
    Assay Technology
    HTRF
    Brand
    Tag-Lite
    Quantity
    1
    Shipping Conditions
    Shipped in Dry Ice
    Therapeutic Area
    Metabolism/Diabetes
    Neuroscience
    Oncology & Inflammation
    Unit Size
    5,000 Assay Points
  • Assay principle

    Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

    gpcr-how-it-works-assay-principle-receptor-binding-l0002red
    Saturation binding (KD)

    A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

    gpcr-how-it-works-saturation-binding-kd-l0002red
    how-it-works-dopamine-d2-receptor-red-antago
    Competitive binding (KI)

    A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.

    gpcr-how-it-works-competitive-binding-ki-l0002red
    how-it-works-dopamine-d2-receptor-red-antago

     

Resources

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.