HTRF VHH XL665-Conjugate, 5,000 Assay Points

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HTRF VHH XL665-Conjugate, 5,000 Assay Points

HTRF Anti-VHH-XL665 can be used to capture alpaca and llama VHH nanobodies.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature	Specification
Application	Protein-Protein Interaction

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HTRF Anti-VHH-XL665 can be used to capture alpaca and llama VHH nanobodies.

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Product Variants

Unit Size: 5,000 Assay Points

Part #:

610VHHXLA

List Price

USD 650.00

Unit Size: 20,000 Assay Points

Part #:

610VHHXLB

List Price

USD 2,079.00

Product information

Overview

Monoclonal anti-VHH antibody has been labeled with XL665 and binds to the native form of both alpaca and llama VHH, as well as recombinant nanobodies. The binding is rapid and stable, making it an ideal choice for use in a variety of assays including nanobody selection and titration.

Specifications

Other Specifications

Application	Protein-Protein Interaction
Brand	HTRF
Detection Modality	HTRF
Product Group	Fluorescent Reagent
Shipping Conditions	Shipped Ambient
Target Class	Binding Assay
Technology	TR-FRET
Unit Size	5,000 Assay Points

How it works

Assay principle

In an HTRF interaction assay, one partner is labeled (directly or indirectly) with the donor, and the other with the acceptor (again, directly or indirectly). The intensity of the resulting signal is proportional to the binding of the 2 partners. In the example shown here, anti-VHH-XL665 binds to the nanobodies raised against protein A, while partner A* binds to a specific Ab labeled with an HTRF donor.

*partner A can also be biotinylated, tagged, or Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with a donor for the detection.

Assay protocol

The example on the right describes the protocol using a 20 µL final assay volume for the detection of an interaction between a nanobody raised against protein A and a non-tagged partner A*. Dispense the two partners (10 µL): i.e. protein A+nanobody, add anti-partner A labeled with donor (5 µL), and anti-VHH-XL665 (5 µL) Then incubate, and finally read.

*partner A can also be biotinylated, tagged or Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin), labeled with a donor for the detection.