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HTRF Tag-Lite Angiotensin Receptors Red ago. Ligand, 5,000 Assay Points
HTRF Tag-Lite Angiotensin Receptors Red ago. Ligand, 5,000 Assay Points
HTRF Angiotensin AT2R receptors red ago vial
This Angiotensin receptor red agonist is an angiotensine II derivative labeled with a red emitting HTRF fluorescent probe.
For research use only. Not for use in diagnostic procedures.
| Feature | Specification |
|---|---|
| Application | Receptor-Ligand Binding |
This Angiotensin receptor red agonist is an angiotensine II derivative labeled with a red emitting HTRF fluorescent probe.
For research use only. Not for use in diagnostic procedures.
Product Variant
Unit Size: 5,000 Assay Points
Part #:
L0007RED
List Price
USD 2,254.00
HTRF Tag-Lite Angiotensin Receptors Red ago. Ligand, 5,000 Assay Points
HTRF Angiotensin AT2R receptors red ago vial
HTRF Tag-Lite Angiotensin Receptors Red ago. Ligand, 5,000 Assay Points
Product information
Overview
Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.
This Angiotensin receptor red agonist is an angiotensine II derivative labeled with a red emitting HTRF fluorescent probe.
This ligand is best suited for use in the Angiotensin AT2 receptor binding assay.
Specifications
| Application |
Receptor-Ligand Binding
|
|---|---|
| Brand |
Tag-lite
|
| Detection Modality |
HTRF
|
| Product Group |
Fluorescent Reagent
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
GPCR
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
| Unit Size |
5,000 Assay Points
|
Video gallery
HTRF Tag-Lite Angiotensin Receptors Red ago. Ligand, 5,000 Assay Points
HTRF Tag-Lite Angiotensin Receptors Red ago. Ligand, 5,000 Assay Points
How it works
Assay principle
Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Resources
Are you looking for resources, click on the resource type to explore further.
Technical Note
A simple method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
Flyer
Tag-lite: the binding receptor assay portfolio addressing the adenosine receptor family
Tag-lite™ is a cell-based, no-wash alternative to traditional radioisotope assays for studying GPCR-ligand interaction.
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