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HTRF Tag-Lite 5HT4 Receptor Red Agonist Ligand, 5,000 Assay Points
This 5HT4 receptor red agonist is an ML10302 derivative labeled with a red emitting HTRF fluorescent probe.
For research use only. Not for use in diagnostic procedures.
Product information
Overview
Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.
This 5HT4 receptor red agonist is an ML10302 derivative labeled with a red emitting HTRF fluorescent probe.
This ligand is best suited for use in the Serotonin 5HT4 receptor binding assay.
Specifications
| Assay Points |
1
|
|---|---|
| Assay Target Type |
Fluorescent reagent
|
| Assay Technology |
HTRF
|
| Brand |
Tag-Lite
|
| Quantity |
1
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
| Unit Size |
1 Item
|
Video gallery
How it works
Assay principle
Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Resources
Flyer
Download your tag-lite brochure
Tag-lite™ is a cell-based, no-wash alternative to traditional radioisotope assays for studying GPCR-ligand interaction.
Adenosine...
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
SDS, COAs, Manuals and more
Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.
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