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HTRF Tag-Lite 5HT4 Receptor Red Agonist Ligand, 5,000 Assay Points

This 5HT4 receptor red agonist is an ML10302 derivative labeled with a red emitting HTRF fluorescent probe.

For research use only. Not for use in diagnostic procedures.

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Feature Specification
Application Receptor-Ligand Binding

This 5HT4 receptor red agonist is an ML10302 derivative labeled with a red emitting HTRF fluorescent probe.

For research use only. Not for use in diagnostic procedures.

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variant
Unit Size: 5,000 Assay Points
Part #:
L0043RED
List Price
USD 3,510.00

Overview

Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.

This 5HT4 receptor red agonist is an ML10302 derivative labeled with a red emitting HTRF fluorescent probe.

This ligand is best suited for use in the Serotonin 5HT4 receptor binding assay.

Specifications

Application
Receptor-Ligand Binding
Brand
Tag-lite
Detection Modality
HTRF
Product Group
Fluorescent Reagent
Shipping Conditions
Shipped in Dry Ice
Target Class
GPCR
Technology
TR-FRET
Therapeutic Area
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
Unit Size
5,000 Assay Points

Video gallery

How it works

Assay principle

Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

gpcr-how-it-works-assay-principle-receptor-binding-l0043red
Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

gpcr-how-it-works-saturation-binding-kd-l0043red
how-it-works-serotonin-5ht4-receptor-red-agonist
Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.

gpcr-how-it-works-competitive-binding-ki-l0043red
how-it-works-serotonin-5ht4-receptor-red-agonist

 

Resources

Are you looking for resources, click on the resource type to explore further.

1-3 of 3 Resources
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Technical Note
A simple method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay
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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

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Flyer
Tag-lite: the binding receptor assay portfolio addressing the adenosine receptor family

Tag-lite™ is a cell-based, no-wash alternative to traditional radioisotope assays for studying GPCR-ligand interaction.

Adenosine...

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