HTRF Tag-Lite 5HT1A Receptor Red Antagonist Ligand, 5,000 Assay Points

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HTRF Tag-Lite 5HT1A Receptor Red Antagonist Ligand, 5,000 Assay Points

SDS

TDS

IFUs, Manuals & more

This 5HT1A receptor red antagonist is a NAN190 derivative labeled with a red emitting HTRF fluorescent probe.

Product information

For research use only. Not for use in diagnostic procedures.

SDS

TDS

IFUs, Manuals & more

Product information

Overview

Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.

This 5HT1A receptor red antagonist is a NAN190 derivative labeled with a red emitting HTRF fluorescent probe.

This ligand is best suited for use in the Serotonin 5HT1A receptor binding assay.

Specifications

Other Specifications

Assay Points	1
Assay Technology	HTRF
Brand	Tag-Lite
Product Group	Fluorescent Reagent
Quantity	1
Shipping Conditions	Shipped in Dry Ice
Therapeutic Area	Cardiovascular Infectious Diseases Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size	1 Item

Video gallery

How it works

Assay principle

Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

How it works serotonin 5ht1a receptor red antagonist 1

Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.