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Cellometer Spectrum Image Cytometry System
The Cellometer™ Spectrum image cytometer is an advanced, modular cell counter capable of running cell-based assays utilizing fluorescent reagents and kits. It can be customized for specific assays/reagents of interest.
For research use only. Not for use in diagnostic procedures.
Product information
Overview
The Cellometer Spectrum image cytometer is a customizable cell counting and cytometry instrument that provides flow-like data with predesigned templates.
You can quickly plot cell population data as a histogram, scatter plot, dot plot or contour plot using FCS express. The filter sets are user-changeable, allowing you to customize the instrument for specific assays/reagents of interest.
Integrated cell analysis solution
Basic cell counting, primary cell viability, and cell-based assays.
- PBMCs
- Stem Cells
- Adipocytes
- Neural Cells
- Hepatocytes
- Dendritic Cells
- Epithelial Cells
- Keratinocytes
- Lymphocytes
- Splenocytes
- Monocytes
- Yeast
- Leukocytes
- Cell Lines
- Nucleated Cells
Dual-fluorescence for accurate primary cell viability
No interference from red blood cells. Analyze bone marrow, peripheral blood, and cord blood without lysing.
User changeable fluorescence filters
Choose from six color options to run two-color assays
Unique algorithms for advanced cell analysis
Determine concentration and viability of hepatocytes, adipocytes, and other sophisticated cell types.
Fast results
Obtain cell images, counts, size measurements and viability calculations in < 30 seconds per sample.
Simple cell-based assays
- Small 20 µl sample size
- Cell count, concentration and viability
- Two-color antibody assays
- GFP/RFP Transfection
- Cell health and cell-based assays, including:
- Apoptosis
- Cell proliferation
- Cell cycle
- Mitochondrial potential
- Phagocytosis
- Surface Marker Analysis
Additional product information
Advantages of Cellometer Spectrum
View Cell Images
- Visually check cell morphology
- Ensure only cells of interest are counted
- Archive and re-analyze cell images
- Export images for publication
Proprietary Pattern-Recognition Software
- Count individual cells in clusters
- Count irregular-shaped cells
- Count cells based on size
- Eliminate debris from cell counts
Non-Fluidic Platform
- Disposable counting chambers – no washing
- Compatible with fragile cells
- Maintenance-free
- Robust optics modules and LED light sources
IQ/OQ Validation and GMP/GLP Accessories
- Installation Qualification reagents/protocol
- Operational Qualification reagents/protocol
- On-site IQ and OQ performance
Fluorescent Cell Counting
- Automatic identification of Acridine Orange (AO) stained cells (shown above) and Propidium Iodine (PI) stained cells (shown below).
- Image and count up to 2×107cells/mL
User-Changeable Fluorescence Optics Modules*
The Spectrum is designed to hold two user-changeable fluorescence optics modules. Purchase only the modules you need and easily configure the instrument by quickly changing the colors to fit your experimental design.
| S1-452-365 Ex. 370 nm (BW: 36 nm) Em: 452 nm (BW: 45 nm) |
BV421 V450 Pacific Blue |
Hoechst 33342 DAPI ViaStain™ Dead Cell Nuclear Blue |
Calcein AM Violet CTV (CellTrace Violet) Tracer Blue BFP |
|
| S1-534-470 Ex: 475 nm (BW: 42 nm) Em: 534 nm (BW:42 nm) |
FITC AlexaFluor® 488 |
AO (acridine orange) SYTO®9, SYTO®13 SYTOX®Green SYTO®BC |
GFP YFP Calcein AM CFSE JC-1 |
|
| S1-594-470 Ex. 475 nm (BW: 42 nm) Em: 594 nm (LP - Long Pass) |
Chlorophyll A Chlorophyll B |
|||
| S1-605-527 Ex: 525 nm (BW: 45 nm) Em: 605 nm (BW: 64 nm) |
AlexaFluor® 546 AlexaFluor® 555 Cy3® PE (R-phycoerythrin) RFP |
PI (propidium iodide) EB (ethidium bromide) SYTOX® Orange |
RFP mCherry TdTomato TurboRed TMRE/TMRM JC-1 |
|
| S1-655-527 Ex: 525 nm (BW: 45 nm) Em: 655 nm (BW: 40 nm) |
PI (propidium iodide) EB (ethidium bromide) 7-AAD |
Nile Red | ||
| S1-692-620 Ex: 628 nm (BW: 40 nm) Em: 692 nm (BW: 40 nm) |
AlexaFluor® 647 APC (allophycocyanin) Cy5® |
SYTOX® Red | iRFP670 CellTrace Far Red Cell Tracker Deep Red LysoTracker Deep Red |
*This table is a partial list of compatible fluorophores, nucleic acid stains, and fluorescent proteins. Please contact Revvity technical support regarding compatibility of other reagents. Sytox, AlexaFluor, and Cy are trademarks of Life Technologies.
Cellometer Spectrum Specifications
| Includes |
FCS Express 6 analysis software (2 licenses) Two Fluorescent Optics Modules Power Supply USB 2.0 Connection Cable Phone/online applications support during set-up |
| Magnification Selection |
5x (ex. most mammalian cells) 10x (ex. yeast, platelets, algae) |
| Imaging Performance |
5x magnifcation Cell Size: 0.5 - 25 microns, Conc. Range: 105 - 107 cells/ml brightfield imaging, fluorescent imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells.* Cellometer CHT4-PD300 Slides are required for cells > 80 microns in diameter |
| Instrument Specifications |
Weight: 24.0 lbs. (10.9 kg) Input to Power Adapter: 100-240 VAC, 50/60 Hz, 1.0A Output to Instrument: 12 VDC, 3.34A |
| PC / Laptop Minimum Requirements (If purchasing Cellometer without PC laptop) |
Windows 10 Intel® i5 (1.6 - 4.40 GHz) Processor 10 Core 4 GB RAM Integrated Graphics Card 1080p Display Resolution 500 GB Hard Drive USB 2.0 Port |
Specifications
| Brand |
Cellometer
|
|---|---|
| Unit Size |
1 unit
|
Resources
Application Note
A novel method for T-cell immunophenotyping using the Cellometer Spectrum image cytometer.
Application Note
A novel NK-cell mediated cytotoxicity detection method using the Cellometer image cytometer.
Technical Note
Comparing fluorescence-based viability detection methods using the Cellometer Vision automated cell counter.
SDS, COAs, Manuals and more
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