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AlphaScreen cAMP Detection Kit, 1,000 Assay Points
AlphaScreen cAMP Detection Kit, 1,000 Assay Points
AlphaScreen no-wash assay kit for quantitative detection of cAMP.
March lab savings - buy on Revvity.com or Revvity punchout to SAVE 10% with promo code!
MARCH10
Sale ends 3/24. Terms and conditions apply.
| Feature | Specification |
|---|---|
| Application | Second Messenger Detection |
| Sample Volume | 10 µL |
AlphaScreen no-wash assay kit for quantitative detection of cAMP.
March lab savings - buy on Revvity.com or Revvity punchout to SAVE 10% with promo code!
MARCH10
Sale ends 3/24. Terms and conditions apply.
AlphaScreen cAMP Detection Kit, 1,000 Assay Points
AlphaScreen cAMP Detection Kit, 1,000 Assay Points
Product information
Overview
AlphaScreen no-wash assay kit for quantitative detection of cAMP. In this AlphaScreen competition assay, a biotinylated cAMP tracer binds to Streptavidin-coated Donor beads, and interacts with anti-cAMP antibody conjugated to AlphaScreen Acceptor beads. When no cAMP is present, the beads come into close proximity. The excitation of the Donor beads triggers the release of singlet oxygen molecules, which diffuse to the Acceptor beads, resulting in light emission at 520-620 nm. With increasing concentrations of endogenous cAMP, fewer Donor and Acceptor beads are brought together, resulting in a decrease in signal.
This kit contains enough reagents to run 1,000 wells using a 25 µL reaction volume. Protocol can be found on the Certificate of Analysis.
This product replaces 6760625D.
Specifications
| Application |
Second Messenger Detection
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaScreen
|
| Detection Modality |
Alpha
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
cAMP
|
| Target Class |
GPCR
|
| Technology |
Alpha
|
| Therapeutic Area |
Metabolic
|
| Unit Size |
1,000 assay points
|
Video gallery
AlphaScreen cAMP Detection Kit, 1,000 Assay Points
AlphaScreen cAMP Detection Kit, 1,000 Assay Points
Citations
Resources
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