Skip to main content
Menu
US

AlphaLISA Human CD80 Detection Kit, 100 Assay Points

The AlphaLISA™ immunoassay kit for human CD80 enables the quantitative determination of human cluster of differentiation 80 (CD80) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Feature Specification
Application Protein Quantification
Dynamic Range 36 - 300,000 pg/mL
Limit of Detection 36 pg/mL  
Limit of Quantification 126 pg/mL  
Sample Volume 10 µL

The AlphaLISA™ immunoassay kit for human CD80 enables the quantitative determination of human cluster of differentiation 80 (CD80) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 100 Assay Points
Part #:
AL3055HV
List Price
USD 1,181.00
Unit Size: 500 Assay Points
Part #:
AL3055C
List Price
USD 2,833.00
Unit Size: 5,000 Assay Points
Part #:
AL3055F
List Price
USD 22,660.00

Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Cluster of differentiation 80 (CD80), also known as B7-1, is a protein that is activated by the binding of CD28 or CTLA-4, providing a costimulatory signal necessary for T cell activation and survival. This protein is expressed on dendritic cells, macrophages, and activated B cells. It has been shown that CD80 and CD86 can substitute for one another in the initial activation of resting CD4(+) T cells as well as the maintenance of their proliferative response. Studies show that increased levels of CD80 in urinary excretion have been linked to Fabry disease, and Minimal Change Disease. This kit is designed to detect and quantify the levels of CD80 in cell culture supernatant and serum.

Specifications

Application
Protein Quantification
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Dynamic Range
36 - 300,000 pg/mL
Limit of Detection
36 pg/mL  
Limit of Quantification
126 pg/mL  
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Blue Ice
Target
CD80
Target Class
Biomarkers
Target Species
Human
Technology
Alpha
Therapeutic Area
Oncology
Unit Size
100 Assay Points

Image gallery

Video gallery

Resources

Are you looking for resources, click on the resource type to explore further.

1-2 of 2 Resources
Application Note Icon
Application Note
Protocol optimization for detection and quantification of membrane-bound proteins using AlphaLISA technology

Membrane proteins, which are key in cellular signaling and often serve as disease biomarkers, are a significant focus in research...

Flyer Icon
Flyer
Reagent solutions for autoimmunity research.

Advance your autoimmune disease research and benefit from Revvity broad offering of reagent technologies

Scroll Icon