AlphaLISA Target Engagement (TE) Buffers can be used to prepare cellular lysates for cell-based target engagement assays. All Buffers are supplied at 5X concentration and should be diluted before use in the target engagement assays.
AlphaLISA Target Engagement (TE) Buffers can be used to prepare cellular lysates for cell-based target engagement assays. All Buffers are supplied at 5X concentration and should be diluted before use in the target engagement assays.
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All AlphaLISA TE Cell Lysis Buffers are supplied at 5X concentration.
AlphaLISA TE Cell Lysis Buffer 1: This formulation is the most aggressive detergent formulation, containing divalent cation chelators, low salt concentration, moderate glycerol concentration.
AlphaLISA TE Cell Lysis Buffer 2: Less aggressive detergent formulation, optimized for lysis of a broad range of cells without releasing nuclear DNA and minimally disrupting protein interactions; physiological salt concentrations.
AlphaLISA TE Cell Lysis Buffer 3: Medium detergent concentration, strongest pH buffering capacity, no glycerol, low salt, 0.1% casein
AlphaLISA TE Cell Lysis Buffer 4: Medium-High detergent concentration, contains divalent cation chelators, close to physiological osmotic strength, no glycerol
AlphaLISA TE Cell Lysis Buffer 5: Medium-High detergent concentration, contains divalent cation chelator, close to physiological osmotic strength, highest glycerol concentration
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA
|
| Buffer/Solvent |
Buffer 2
|
| Detection Modality |
Alpha
|
| One Unit Contains |
Single Buffer
|
| Product Group |
Buffer
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target Class |
Phosphoproteins
|
| Technology |
Alpha
|
| Unit Size |
100 mL
|
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