AlphaLISA Target Engagement (TE) Buffers can be used to prepare cellular lysates for cell-based target engagement assays.
AlphaLISA Target Engagement (TE) Buffers can be used to prepare cellular lysates for cell-based target engagement assays.
AlphaLISA TE Cell Lysis Buffer 1: This formulation is the most aggressive detergent formulation, containing divalent cation chelators, low salt concentration, moderate glycerol concentration.
AlphaLISA TE Cell Lysis Buffer 2: Less aggressive detergent formulation, optimized for lysis of a broad range of cells without releasing nuclear DNA and minimally disrupting protein interactions; physiological salt concentrations.
AlphaLISA TE Cell Lysis Buffer 3: Medium detergent concentration, strongest pH buffering capacity, no glycerol, low salt, 0.1% casein
AlphaLISA TE Cell Lysis Buffer 4: Medium-High detergent concentration, contains divalent cation chelators, close to physiological osmotic strength, no glycerol
AlphaLISA TE Cell Lysis Buffer 5: Medium-High detergent concentration, contains divalent cation chelator, close to physiological osmotic strength, highest glycerol concentration
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA
|
Buffer/Solvent |
Buffer 1
|
Detection Modality |
Alpha
|
One Unit Contains |
Single Buffer
|
Product Group |
Buffer
|
Shipping Conditions |
Shipped in Blue Ice
|
Target Class |
Phosphoproteins
|
Technology |
Alpha
|
Unit Size |
100 mL
|
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