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AlphaLISA SureFire Ultra Human and Mouse Total Wee1 Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Total Wee1 assay is a sandwich immunoassay for quantitative detection of total Wee1 in cellular lysates using Alpha Technology.

Part Number: ALSU-TWEE-A500
Unit Size:
Part Number: ALSU-TWEE-A10K
Unit Size:
Part Number: ALSU-TWEE-A50K
Unit Size:
Part Number: ALSU-TWEE-A-HV
Unit Size:
  • Wee1, a serine/threonine kinase, plays a key role in regulating the cell cycle. Wee1 activity is increased in response to DNA damage to support cell cycle arrest. By phosphorylating and deactivating CDK1, Wee1 controls the G2/M cell cycle checkpoint. Wee1 phosphorylates CDK2 during the S phase, preventing cells from moving on to the subsequent cell cycle phase until DNA replication or repair is complete. Wee1 is overexpressed in numerous tumors and has been linked to cancer patients' poor disease-free survival. Thus, Wee1 inhibition is a current research topic, especially with the introduction of novel strategies utilizing PROTACs (PROteolysis TArgeting Chimeras).

    The AlphaLISA SureFire Human and Mouse Total Wee1 Detection Kit is a sandwich immunoassay for the quantitative detection of total Wee1 in cellular lysates, using Alpha Technology.

    Formats:
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with:
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for:
    • Cellular kinase assays
    • Receptor activation studies
    • Screening

    For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Mouse
    Therapeutic Area
    Oncology