Skip to main content
Menu
US

AlphaLISA SureFire Ultra Human Phospho-TFEB (Ser211) Detection Kit, 100 Assay Points

The AlphaLISA™ SureFire® Ultra™ Human Phospho-TFEB (Ser211) assay is a sandwich immunoassay for quantitative detection of phospho-TFEB (Ser211) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Feature Specification
Application Cell Signaling
Sample Volume 30 µL

The AlphaLISA™ SureFire® Ultra™ Human Phospho-TFEB (Ser211) assay is a sandwich immunoassay for quantitative detection of phospho-TFEB (Ser211) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 500 Assay Points
Part #:
ALSU-PTFEB-B500
List Price
USD 2,346.00
Unit Size: 10,000 Assay Points
Part #:
ALSU-PTFEB-B10K
List Price
USD 14,270.00
Unit Size: 50,000 Assay Points
Part #:
ALSU-PTFEB-B50K
List Price
USD 46,060.00
Unit Size: 100 Assay Points
Part #:
ALSU-PTFEB-B-HV
List Price
USD 694.44

Overview

Transcription factor EB (TFEB) plays a key role in the regulation of basic cellular processes, including lysosomal biogenesis and autophagy. Dephosphorylated TFEB travels to the nucleus to trigger the transcription of target genes while phosphorylated TFEB exists in the cytoplasm. Mechanistic target of rapamycin (mTOR)-mediated phosphorylation, which takes place at the lysosomal surface, controls the subcellular location and function of TFEB. TFEB is implicated in various diseases, such as lysosomal storage disorders, neurodegenerative diseases, cancers, and metabolic disorders.

The AlphaLISA SureFire Human Phospho-TFEB (Ser211) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-TFEB (Ser211) in cellular lysates, using Alpha Technology.

Formats
  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies
Alpha SureFire kits can be used for
  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Phosphorylation
Product Group
Kit
Sample Volume
30 µL
Shipping Conditions
Shipped in Blue Ice
Target
TFEB
Target Class
Phosphoproteins
Target Species
Human
Technology
Alpha
Unit Size
100 Assay Points

Image gallery

Video gallery

Resources

Are you looking for resources, click on the resource type to explore further.

1-4 of 4 Resources
Guide Icon
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Brochure Icon
Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

Application Note Icon
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...

Application Note Icon
Application Note
Characterizing chemokine receptor inhibitors with AlphaLISA SureFire Ultra, Alpha SureFire Ultra Multiplex and LANCE Ultra cAMP assays

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...

Scroll Icon