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AlphaLISA SureFire Ultra Human Phospho-TFEB (Ser211) Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra Human Phospho-TFEB (Ser211) assay is a sandwich immunoassay for quantitative detection of phospho-TFEB (Ser211) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PTFEB-B500
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Part Number: ALSU-PTFEB-B10K
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Part Number: ALSU-PTFEB-B50K
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Part Number: ALSU-PTFEB-B-HV
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  • Transcription factor EB (TFEB) plays a key role in the regulation of basic cellular processes, including lysosomal biogenesis and autophagy. Dephosphorylated TFEB travels to the nucleus to trigger the transcription of target genes while phosphorylated TFEB exists in the cytoplasm. Mechanistic target of rapamycin (mTOR)-mediated phosphorylation, which takes place at the lysosomal surface, controls the subcellular location and function of TFEB. TFEB is implicated in various diseases, such as lysosomal storage disorders, neurodegenerative diseases, cancers, and metabolic disorders.

    The AlphaLISA SureFire Human Phospho-TFEB (Ser211) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-TFEB (Ser211) in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Detection
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human

Resources

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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
surefire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx