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AlphaLISA SureFire Ultra Human Phospho-Tau (Ser404) Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra Human Phospho-Tau (Ser404) assay is a sandwich immunoassay for quantitative detection of phospho-Tau (Ser404) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PTAU-C50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-PTAU-C10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-PTAU-C-HV
Unit Size: 100 Assay Points
Part Number: ALSU-PTAU-C500
Unit Size: 500 Assay Points
  • Tau, also known as MAPT (Microtubule Associated Protein Tau), is a phosphoprotein that is mostly produced in neurons of the central nervous system and is involved in microtubule construction and stability. Around 85 phosphorylation sites are present in Tau, and numerous kinases, including CDK5, MAP kinase, and GSK3 (Tau's primary kinase), can phosphorylate these sites. Numerous neurodegenerative diseases, including Tauopathy, Parkinson's, and Alzheimer's, are characterized by Tau hyperphosphorylation and aggregation.

    The AlphaLISA SureFire Human Phospho-Tau (Ser404) Detection Kit is a sandwich immunoassay for the quantitative detection of phosphorylated tau Ser404 in cellular lysates, using Alpha Technology.

    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
    • Replacing a Tau ELISA
  • Application
    Protein Detection
    Assay Target
    Assay Technology
    Automation Compatible
    AlphaLISA SureFire Ultra
    Detection Method
    Experimental Type
    In vitro
    Target Species
    Therapeutic Area
    Unit Size
    50,000 Assay Points


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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
SureFire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx