Tau, also known as MAPT (Microtubule Associated Protein Tau), is a phosphoprotein that is mostly produced in neurons of the central nervous system and is involved in microtubule construction and stability. Around 85 phosphorylation sites are present in Tau, and numerous kinases, including CDK5, MAP kinase, and GSK3 (Tau's primary kinase), can phosphorylate these sites. Numerous neurodegenerative diseases, including Tauopathy, Parkinson's, and Alzheimer's, are characterized by Tau hyperphosphorylation and aggregation.

The AlphaLISA SureFire Human Phospho-Tau (Ser404) Detection Kit is a sandwich immunoassay for the quantitative detection of phosphorylated tau Ser404 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening
• Replacing a Tau ELISA