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AlphaLISA SureFire Ultra Human and Mouse Phospho-RAB 8A (Thr72) Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Phospho-RAB 8A (Thr72) assay is a sandwich immunoassay for quantitative detection of phospho-RAB 8A (Thr72) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PRAB8-B500
Unit Size: 500 Assay Points
Part Number: ALSU-PRAB8-B10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-PRAB8-B50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-PRAB8-B-HV
Unit Size: 100 Assay Points
  • Ras-related protein RAB8A is a member of the RAS superfamily, which consists of small GTP/GDP binding proteins. Small GTPases Rab are key regulators of intracellular membrane trafficking, including the formation of transport vesicles to their fusion with membranes. RAB8A is involved in polarized vesicular trafficking and neurotransmitter release. RAB8A is associated with selective autophagy. Rab8a is one of several Rab GTPases that are substrates of leucine-rich repeat kinase 2 (LRRK2), a serine/threonine kinase that is linked to Parkinson's disease.

    The AlphaLISA SureFire Human and Mouse Phospho-RAB 8A (Thr72) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-RAB 8A (Thr72) in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Detection
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Shipping Conditions
    Shipped in Blue Ice
    Target Species
    Human
    Mouse
    Therapeutic Area
    Neuroscience
    Unit Size
    50,000 Assay Points

Resources

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Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...