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AlphaLISA SureFire Ultra Human and Mouse Phospho-RAB 8A (Thr72) Detection Kit, 500 Assay Points

The AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-RAB 8A (Thr72) assay is a sandwich immunoassay for quantitative detection of phospho-RAB 8A (Thr72) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Product Variants
Part number: ALSU-PRAB8-B500
Unit Size: 500 Assay Points
List price: USD 2,346.00
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USD 2,346.00
USD 2,346.00 /each
Part number: ALSU-PRAB8-B10K
Unit Size: 10,000 Assay Points
List price: USD 14,270.00
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USD 0.00
USD 14,270.00 /each
Part number: ALSU-PRAB8-B50K
Unit Size: 50,000 Assay Points
List price: USD 46,060.00
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USD 0.00
USD 46,060.00 /each
Part number: ALSU-PRAB8-B-HV
Unit Size: 100 Assay Points
List price: USD 694.44
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USD 0.00
USD 694.44 /each

Overview

Ras-related protein RAB8A is a member of the RAS superfamily, which consists of small GTP/GDP binding proteins. Small GTPases Rab are key regulators of intracellular membrane trafficking, including the formation of transport vesicles to their fusion with membranes. RAB8A is involved in polarized vesicular trafficking and neurotransmitter release. RAB8A is associated with selective autophagy. Rab8a is one of several Rab GTPases that are substrates of leucine-rich repeat kinase 2 (LRRK2), a serine/threonine kinase that is linked to Parkinson's disease.

The AlphaLISA SureFire Human and Mouse Phospho-RAB 8A (Thr72) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-RAB 8A (Thr72) in cellular lysates, using Alpha Technology.

Formats
  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies
Alpha SureFire kits can be used for
  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Application
Cell Signaling
Assay Target
RAB 8A
Assay Target Class
Phosphoproteins
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Method
Alpha
Molecular Modification
Phosphorylation
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Blue Ice
Target Species
Human
Mouse
Therapeutic Area
Neuroscience
Unit Size
500 Assay Points

Video gallery

Resources

1-4 of 4 Resources
Guide Icon
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...

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Application Note
Characterizing chemokine receptor inhibitors with AlphaLISA SureFire Ultra, Alpha SureFire Ultra Multiplex and LANCE Ultra cAMP assays

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...

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