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AlphaLISA Surefire Ultra Human, Mouse, and Rat Phospho-JNK1/2/3 (Thr183/Tyr185) Detection Kit, 500 Assay Points
The AlphaLISA™ SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product information
Overview
The AlphaLISA™ SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology.
In the AlphaLISA™ SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA™ SureFire® Ultra™ kits are compatible with:
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire® kits can be used for:
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Kinase
Protein Analysis & Detection
|
|---|---|
| Assay Pathway |
MAPK
|
| Assay Points |
500
|
| Assay Target |
JNK1/2/3
|
| Assay Target Class |
Phospho-protein
|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Method |
Alpha
|
| Experimental Type |
In vitro
|
| Format |
Microplates
|
| Quantity |
1
|
| Quantity in a Package Amount |
1.0 Units
|
| Second Messenger Release |
Protein phosphorylation
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Therapeutic Area |
Central Nervous System
Metabolic
|
| Unit Size |
500 Assay Points
|
Image gallery
Video gallery
Resources
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
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