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AlphaLISA Surefire Ultra Human, Mouse, and Rat Phospho-JNK1/2/3 (Thr183/Tyr185) Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PJNK-A500
Unit Size: 500 Assay Points
Part Number: ALSU-PJNK-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-PJNK-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-PJNK-A-HV
Unit Size: 100 Assay Points
  • The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology.

    In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

    AlphaLISA® SureFire® Ultra™ kits are compatible with:

    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies

    Alpha SureFire® kits can be used for:

    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Automation Compatible
    Yes
    Application
    Kinase
    Protein Analysis & Detection
    Assay Points
    10000
    Assay Target
    JNK1/2/3
    Assay Target Class
    Phospho-protein
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Second Messenger Release
    Protein phosphorylation
    Therapeutic Area
    Central Nervous System
    Metabolic
    Assay Pathway
    MAPK
    Brand
    AlphaLISA SureFire Ultra
    Quantity
    1
    Quantity in a Package Amount
    1.0 Units
    Unit Size
    10,000 Assay Points

Resources

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Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
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Reagent solutions for autoimmunity research.
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