Glycogen synthase kinase-3 beta (GSK3β) is active in numerous central intracellular signaling pathways and regulates important cellular processes, from proliferation and immune response to glucose regulation and apoptosis. GSK3 kinase phosphorylation inhibits the activity of downstream targets such as β-catenin. GSK3 is regulated by cellular localization and by protein complex formation in the insulin pathway. Insulin activates PI3K, which phosphorylates AKT and in turn, activated AKT phosphorylates GSK3α on Ser21 and GSK3β on Ser9, resulting in GSK3 inactivation and inducing activation of glycogen synthase by dephosphorylation. GSK3 is considered a versatile target for various disease research applications, including oncology, neurological disorders, and diabetes.

The AlphaLISA SureFire Human and Mouse Total GSK3β Detection Kit is a sandwich immunoassay for the quantitative detection of total GSK3β in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening