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AlphaLISA Surefire Ultra Human Phospho-CD3 ζ (Tyr142) Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-CD3 ζ (Tyr142) assay. This control is already provided in the AlphaLISA SureFire UltraPhospho-CD3 ζ (Tyr142) assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Part number: ALSU-PCD3-B-L
Unit Size: 1 Vial
List price: USD 142.66
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USD 142.66
USD 142.66 /each

Overview

This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-CD3 ζ (Tyr142) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Jurkat cells were seeded at 500 K/mL and grown overnight in T175 flasks in 100 mL of 10% FBS containing medium. Cells were washed in HBSS + 0.1% BSA, adjusted to 3.5 X 106 cells/mL and treated with 44 mM H2O2 for 10 minutes. Cells were lysed with SureFire Ultra Lysis Buffer.

Specifications

Application
Cell Signaling
Assay Target
CD3 ζ
Assay Target Class
Phosphoproteins
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Method
Alpha
Molecular Modification
Phosphorylation
Product Group
Control Lysate
Shipping Conditions
Shipped in Blue Ice
Target Species
Human
Therapeutic Area
Autoimmunity
Immuno-oncology
Oncology
Unit Size
1 Vial

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