Skip to main content
Menu
US

AlphaLISA Surefire Ultra Human, Mouse, and Rat Aggregated α-Synuclein Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ alpha synuclein aggregation assay kit can be used to measure levels of aggregated alpha-synuclein in cellular lysates using no-wash Alpha technology. The antibodies recognize distinct epitopes specific to the Aggregated form of α-Synuclein.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: ALSU-AASYN-A500
Unit Size: 500 Assay Points
List price: USD 2,346.00
Your price:
USD 0.00
USD 2,346.00 /each
Part number: ALSU-AASYN-A10K
Unit Size: 10,000 Assay Points
List price: USD 14,270.00
Your price:
USD 0.00
USD 14,270.00 /each
Part number: ALSU-AASYN-A50K
Unit Size: 50,000 Assay Points
List price: USD 46,060.00
Your price:
USD 46,060.00
USD 46,060.00 /each
Part number: ALSU-AASYN-A-HV
Unit Size: 100 Assay Points
List price: USD 694.44
Your price:
USD 0.00
USD 694.44 /each

Overview

α-Synuclein is a neuronal protein encoded by the SNCA gene that regulates synaptic vesicle trafficking and subsequent neurotransmitter release. α-Synuclein is a prominent component of intracellular fibrillary aggregates in the brains of patients suffering from synucleinopathies. This protein represents a key molecular hallmark for Parkinson’s disease, dementia with Lewy bodies, or multiple system atrophy. The extensive phosphorylation of α-Synuclein on Ser129 is also associated with a pathological event.

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA®SureFire®Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA®SureFire®Ultra™ kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Detecting alpha synuclein aggregation
  • Cellular kinase assays
  • Receptor activation studies
  • Screening
  • Replacing an alpha synuclein ELISA kit

Specifications

Application
Protein Analysis & Detection
Assay Points
50000
Assay Target Class
Protein
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Method
Alpha
Therapeutic Area
Central Nervous System
Unit Size
50,000 Assay Points

Image gallery

Video gallery

Resources

1-4 of 4 Resources
Guide Icon
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Brochure Icon
Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

Application Note Icon
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...

Application Note Icon
Application Note
Characterizing chemokine receptor inhibitors with AlphaLISA SureFire Ultra, Alpha SureFire Ultra Multiplex and LANCE Ultra cAMP assays

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

Scroll Icon