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DELFIA Enhancement Solution, 50 mL

DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.

DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.

Product variants
Unit Size: 50 mL
Part #:
1244-104
List price
USD 66.42
Your online price:
Unit Size: 250 mL
Part #:
1244-105
List price
USD 334.26
Your online price:
Unit Size: 1 L
Part #:
4001-0010
List price
USD 1,250.71
Your online price:
For in vitro diagnostic use. This product is only available where licensed in accordance with law. Please contact your local representative for availability.

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Overview

The DELFIA® Enhancement Solution is an acidic chelating detergent solution intended for use in the quantitative determination of Eu3+/Sm3+ when using a time-resolved fluoroimmunoassay. The solution dissociates Eu3+/Sm3+ from solid phase bound Eu-labeled antibodies or proteins during a time period of a few minutes to form a homogeneous and highly fluorescent micellar chelate solution. The solution allows highly sensitive Eu3+/Sm3+ measurements to be made when using the time-resolved fluorometer. The DELFIA® Enhancement Solution is also used as part of Tb3+/Dy3+ measurement together with DELFIA® Enhancer.

Because DELFIA TRF chelates are not intrinsically fluorescent, this solution (an acidic chelating detergent solution) is added to the microplate wells after a wash step to develop the fluorescence signal. This provides highly sensitive Eu and/or Sm measurements. Suitable for use in all multianalyte assays.

Specifications

Automation Compatible
Yes
Brand
DELFIA
Detection Modality
DELFIA
Product Group
Buffer
Regulatory Status
CE-IVD marked
Technology
TRF
Unit Size
50 mL

Resources

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Application Note
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development

DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...

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Application Note
Qualification of a DELFIA Assay for the Detection of Anti-mouse IgG Antibodies in Human Serum

We report here the development of a Dissociation Enhanced Lanthanide Fluorescence Immunoassay (DELFIA) for the detection of ADA in...

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Guide
Time-resolved fluorometry with the DELFIA method user guide

This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.

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