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DELFIA Enhancement Solution, 1,000 mL
DELFIA Enhancement Solution, 1,000 mL
DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
DELFIA Enhancement Solution, 1,000 mL
DELFIA Enhancement Solution, 1,000 mL
Product information
Overview
The DELFIA® Enhancement Solution is an acidic chelating detergent solution intended for use in the quantitative determination of Eu3+/Sm3+ when using a time-resolved fluoroimmunoassay. The solution dissociates Eu3+/Sm3+ from solid phase bound Eu-labeled antibodies or proteins during a time period of a few minutes to form a homogeneous and highly fluorescent micellar chelate solution. The solution allows highly sensitive Eu3+/Sm3+ measurements to be made when using the time-resolved fluorometer. The DELFIA® Enhancement Solution is also used as part of Tb3+/Dy3+ measurement together with DELFIA® Enhancer.
Because DELFIA TRF chelates are not intrinsically fluorescent, this solution (an acidic chelating detergent solution) is added to the microplate wells after a wash step to develop the fluorescence signal. This provides highly sensitive Eu and/or Sm measurements. Suitable for use in all multianalyte assays.
Specifications
| Automation Compatible |
Yes
|
|---|---|
| Brand |
DELFIA
|
| Detection Modality |
DELFIA
|
| Product Group |
Buffer
|
| Technology |
TRF
|
| Unit Size |
1 L
|
Resources
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DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...
This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.
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