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AlphaLISA Assay Buffer (10X), 10 mL

This specially formulated buffer is intended for use in most AlphaLISA immunoassay kits, and can be purchased to develop your own AlphaLISA immunoassay.

This specially formulated buffer is intended for use in most AlphaLISA immunoassay kits, and can be purchased to develop your own AlphaLISA immunoassay.

Product variants
Unit Size: 10 mL
Part #:
AL000C
List price
USD 325.22
Your online price:
Unit Size: 100 mL
Part #:
AL000F
List price
USD 623.43
Your online price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

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Overview

Several pre-formulated buffers are available for Alpha assays. These have have been developed to meet the varying needs of different Alpha assays, and one of them may be a good choice for an assay that you are developing. AlphaLISA Immunoassay Buffer (IAB) is a broadly-applicable buffer, used in most AlphaLISA immunoassays. This buffer ontains detergent and low salt, so it can be used to lyse cells in some assays. It is generally sufficient to expose the protein epitopes of membrane proteins for Alpha assay detection.

10X formulation: 250 mM HEPES, pH 7.4, 1% Casein, 10 mg/mL Dextran-500, 5% Triton X-100 and 0.5% Kathon

Specifications

Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Product Group
Buffer
Shipping Conditions
Shipped in Blue Ice
Technology
Alpha
Unit Size
10 mL

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Technical Note
Developing a high throughput AlphaLISA assay for screening activity of biologics produced by engineered probiotic microbes

Due to limitations driven by circulatory half-life and drug target bioavailability, injected biologics often require the injection...

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