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Immunoassay buffer, 10X, 10 mL

Pre-formulated buffer optimal for most AlphaLISA analyte detection assays. This buffer is used in most AlphaLISA immunoassay kits, and can be purchased to develop your own AlphaLISA immunoassay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: AL000C
Unit Size: 10 mL
List price: USD 315.75
Your price:
USD 315.75
USD 315.75 /each
Part number: AL000F
Unit Size: 100 mL
List price: USD 605.27
Your price:
USD 0.00
USD 605.27 /each

Overview

Several pre-formulated buffers are available for Alpha assays. These have have been developed to meet the varying needs of different Alpha assays, and one of them may be a good choice for an assay that you are developing. AlphaLISA Immunoassay Buffer (IAB) is a broadly-applicable buffer, used in most AlphaLISA immunoassays. This buffer ontains detergent and low salt, so it can be used to lyse cells in some assays. It is generally sufficient to expose the protein epitopes of membrane proteins for Alpha assay detection.

10X formulation: 250 mM HEPES, pH 7.4, 1% Casein, 10 mg/mL Dextran-500, 5% Triton X-100 and 0.5% Proclin-300

Specifications

Application
Protein Analysis & Detection
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Format
Microplates
Unit Size
10 mL

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Application Note
Screening for Inhibitors of PD-1 and PD-L1 Binding with AlphaLISA Technology

Many tumor cells, which under normal, healthy conditions would be recognized by the body’s T cells and thereby targeted for...

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