Skip to main content
Application Note

Optimizing 384-well qPCR plate preparation while decreasing costs

optimizing-384well-app-note-512x288

Quantitative Polymerase Chain Reaction (qPCR) is an important step in many laboratory experiments to accurately quantify nucleic acids in next generation sequencing (NGS) and other applications. Propagation of errors from inaccurate liquid transfers can greatly impact the quality of a qPCR assay.

The Centre for Cancer Biology (SA Pathology and the University of South Australia) compared multiple ways of preparing qPCR plates using:

  • Manual Preparation
  • JANUS® G3 Varispan 4-tip Workstation Single Dispense
  • JANUS G3 Varispan 4-tip Workstation Multiple Dispense
  • Qiagen® QIAgility® Instrument
  • FlexDrop iQ Non-Contact Dispenser (500 μL version)

Read the application note to review the qPCR plate prep data obtained from these different methods.

For research use only. Not for use in diagnostic procedures.

To view the full content please answer a few questions

By submitting my personal data, I acknowledge that Revvity Inc. and its affiliates (“Company”) will process my personal data provided above consistent with the Company’s Privacy Policy available here.

This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Download Resource

Optimizing 384-well qPCR plate preparation while decreasing costs

Download Application Note