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Exosome/cfRNA Analysis

Convenient gel-free workflow for analysis of extracellular vesicles from cfRNA

Extracellular vesicles are membrane-bound compartments which are being increasingly recognized as important players in cell-to-cell communication. They are secreted by most cell types and are present in many and perhaps all body fluids, including plasma. Extracellular vesicles are classified in different types according to their size. The most characterized are exosomes, which are 50-200 nm in size. Exosomes transport a cargo of different proteins and RNA libraries from exoRNA. The amount of RNA obtained from exosomes is low, with just a few picograms corresponding to miRNA. Based on quantitative analysis this can be as little as 1 miRNA molecule/100 exosomes, making this a very challenging sample type.


For research use only. Not for use in diagnostic procedures.

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Gel-free small RNA-seq library prep

The new NEXTFLEX® Small RNA-Seq Kit v4 allows preparation of small RNA libraries ready for sequencing on certain Illumina® platforms in approximately 6 hours with as little input as 1 ng of total RNA. Libraries prepared with this kit have a higher proportion of mapping reads and unique miRNAs than other workflows available in the market.

 

Automation of small RNA-seq workflows

Small RNA library preparation is one of the most difficult types of NGS library preparation, which can be simplified with automation to enable you to improve miRNA discovery.

The NEXTFLEX Small RNA-Seq Kit v4 is a gel-free, fully automatable protocol using an adapter dimer reduction approach. The advantage is that the protocol automated on the Sciclone® G3 NGS/NGSx and Zephyr® G3 NGS workstations enables high sample throughput, generating more libraries in less time while maintaining data quality.

NGS Library Prep Solutions

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Please note that product labeling (such as kit insert, product label, and kit box) may be different compared to the company branding. Please contact your local representative for further details.