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HTRF Phospho-total Lysis Buffer #3 (4X), 130 mL
HTRF Phospho-total Lysis Buffer #3 (4X), 130 mL
Generic HTRF big generic bottle image
Lysis buffer#3 is one of the components for protein extraction provided with HTRF total and phospho kits (cf. lysis buffer compatiblity table). It can be ordered as a separate consumable.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
Lysis buffer#3 is one of the components for protein extraction provided with HTRF total and phospho kits (cf. lysis buffer compatiblity table). It can be ordered as a separate consumable.
Product variant
Unit Size: 130 ml
Part #:
64KL3FDF
List price
USD 617.00
Your online price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Phospho-total Lysis Buffer #3 (4X), 130 mL
Generic HTRF big generic bottle image
HTRF Phospho-total Lysis Buffer #3 (4X), 130 mL
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Product information
Overview
Correct buffer conditions are essential for optimal cell lysis and extraction of proteins. The composition of the phospho / total protein lysis buffer 3 has been carefully optimized for the quantification of protein expression and phosphorylation. You may refer to the lysis buffer compatibility table to find out which assay works best with buffer 3.
HTRF assays offer many advantages over other technologies:
- Homogeneous add-and-read format
- No wash steps
- Low background
- Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
- Stable signal, providing flexibility in time of readout or size of assays
Assay details
Lysis buffer compatibility table
Revvity's portfolio of phospho and total protein assays has been optimized with 4 different lysis buffers. In most instances, one, sometimes two buffers among the four work significantly better for a given assay. You may refer the compatibility table to find out which buffer you should use preferentially with your assay of interest. This table will also allow you to pick a buffer working equally well with several assays, in order to run them using the same lysate.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Buffer
|
| Shipping Conditions |
Shipped Ambient
|
| Target Class |
Phosphoproteins
|
| Technology |
TR-FRET
|
| Unit Size |
130 ml
|
Video gallery
HTRF Phospho-total Lysis Buffer #3 (4X), 130 mL
HTRF Phospho-total Lysis Buffer #3 (4X), 130 mL
Resources
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Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
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