AlphaLISA Mouse IL12p70 Detection Kit, 500 assay points

Mouse IL-12p70 is a heterodimeric pro-inflammatory cytokine composed of the p35 and p40 subunits, and belongs to the IL-12 cytokine family, which also includes IL-23, IL-27, and IL-35. The p35 subunit also associates with EBI3 to form IL-35, whereas the p40 subunit can form IL-23 (in combination with p19) or exist as a p40 homodimer (IL-12p80) or monomer. These different combinations confer distinct and sometimes opposing biological functions within the IL-12 family.

IL-12p70 is primarily produced by antigen-presenting cells such as dendritic cells, macrophages, and neutrophils, and can also be secreted by activated B cells and helper T cells in response to antigenic stimulation and inflammatory signals. Functionally, IL-12 plays a central role in bridging innate and adaptive immunity. It promotes the differentiation of naive CD4⁺ T cells into Th1 cells, enhances interferon-γ (IFN-γ) production, and stimulates the cytotoxic activity of natural killer (NK) cells and CD8⁺ T lymphocytes.

These effects are mediated through binding to the heterodimeric IL-12 receptor (IL-12Rβ1 and IL-12Rβ2), leading to activation of the JAK/STAT signaling pathway, particularly STAT4.

Studying the production and regulation of IL-12p70 during infection is therefore critical for understanding immune defense mechanisms and the orchestration of inflammatory responses.

Formats

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features

• No wash steps, no separation steps
• Alternative to ELISA technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 4 hours

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules, triggering a cascade of energy transfer in the Acceptor beads, and resulting in a sharp peak of light emission at 615 nm.