AlphaLISA Human Fibrin D-dimer Detection Kit, 500 Assay Points

Formats:

• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

D-dimer is a 200 kDa fragment of fibrinogen and is produced during clot lysis. Fibrinogen is synthesized by the liver and is one of the most abundant proteins in plasma. It has two identical subunits of three polypeptide chains (alpha, beta and gamma). In the coagulation process, fibrinogen is cleaved by thrombin to form the fibrin clot. Then the clot is dissolved through plasmin cleavage of fibrin and forms D-dimer. D-dimer is an important cardiovascular biomarker. It is widely used in diagnosis to exclude deep vein thrombosis (DVT) and pulmonary embolism. Its concentration is also increased after surgery, during pregnancy, and in cancer.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.