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LANCE Ultra ULight GT Peptide Substrate, 10nmoles

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Poly Glu-Tyr (4:1) labeled with ULightTM dye. This random polymer is a Tyrosine kinase substrate.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: TRF0100-D
Unit Size: 1 nmol
Part Number: TRF0100-M
Unit Size: 10 nmol
  • LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:

    • Test conditions: Rapid assay setup to move straight into optimized assay performance
    • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing
    • Phospho-motifs: Substrate sequences are provided
    • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

    For ULight substrates:

    • 0.5 nmoles = 1,000 assay points
    • 5 nmoles = 10,000 assay points

    In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.

  • Application
    Kinase
    Automation Compatible
    Yes
    Brand
    LANCE Ultra
    Detection Method
    Time-Resolved Fluorescence (TRF)
    TR-FRET
    Experimental Type
    In vitro
    Fluorophore
    ULight
    Format
    Microplates
    Molecular Modification
    Phosphorylation
    Shipping Conditions
    Shipped in Dry Ice
    Unit Size
    10 nmol