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LANCE Ultra ULight Histone H3 Peptide Substrate, 0,125nmole

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence: ARTKQTARKSTGGK

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: TRF0125-C
Unit Size: 0.125 nmol
List price: USD 418.55
Your price:
USD 418.55
USD 418.55 /each
Part number: TRF0125-D
Unit Size: 0.5 nmol
List price: USD 596.88
Your price:
USD 0.00
USD 596.88 /each
Part number: TRF0125-M
Unit Size: 5 nmol
List price: USD 1,708.00
Your price:
USD 0.00
USD 1,708.00 /each

Overview

LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:

  • Test conditions: Rapid assay setup to move straight into optimized assay performance
  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing
  • Phospho-motifs: Substrate sequences are provided
  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

For ULight substrates:

  • 0.5 nmoles = 1,000 assay points
  • 5 nmoles = 10,000 assay points

In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.

Specifications

Application
Kinase
Automation Compatible
Yes
Brand
LANCE Ultra
Detection Method
Time-Resolved Fluorescence (TRF)
TR-FRET
Experimental Type
In vitro
Fluorophore
ULight
Format
Microplates
Molecular Modification
Phosphorylation
Shipping Conditions
Shipped in Dry Ice
Therapeutic Area
Oncology
Unit Size
0.125 nmol
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