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SureLight Allophycocyanin-anti-6His Antibody, 1 mg

APC-labeled anti-His antibody, for capturing His-tagged proteins and peptides in LANCE TR-FRET assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part number: AD0059H
List price: USD 810.88
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USD 810.88
USD 810.88 /each

Overview

Mouse monoclonal antibody against 6xHis polypeptide conjugated to allophycocyanin (APC). APC is a fluorescent light harvesting protein unique to cyanobacteria and red algae and a member of the phycobiliprotein family of direct fluorescent dyes. Surelight APC is a chemically and intramolecularly cross-linked form of a protein. It is produced using a proprietary manufacturing process to increase thermal stability while maintaining the fluorescent and spectral properties of native dye.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Antibody Conjugates
Anti-6X His
Application
Protein-protein interactions
Automation Compatible
Yes
Brand
LANCE SureLight
Detection Method
TR-FRET
Fluorophore
SureLight APC
Format
Microplates
One Unit Contains
1.0 mg
Shipping Conditions
Shipped in Blue Ice
Unit Size
1 mg

Citations

Resources

1-3 of 3 Resources
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Application Note
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Technical Note
How to access true Kd values in Protein-Protein Interaction assays

Investigate the dynamics of Interactions

Kinetic values are of importance for characterizing protein-protein interactions...

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Guide
The ultimate guide to PPI assays with HTRF

Your dream assay does not exist yet? Create it!

This guide will provide you with tools to help you develop your very own protein...

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