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AlphaScreen Streptavidin Donor Beads, 50 mg

Alpha Donor beads coated with streptavidin, for biotinylated biomolecules for use in Alpha no-wash assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: 6760002S
Unit Size: 1 mg
List price: USD 566.46
Your price:
USD 0.00
USD 566.46 /each
Part number: 6760002
Unit Size: 5 mg
List price: USD 2,263.00
Your price:
USD 0.00
USD 2,263.00 /each
Part number: 6760002B
Unit Size: 50 mg
List price: USD 15,950.00
Your price:
USD 15,950.00
USD 15,950.00 /each

Overview

These beads can be used to capture biotinylated antibodies, proteins, peptides, nucleic acids, and small molecules, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:

  • Protein-protein interaction assays
  • Protein-DNA interaction assays
  • Protein-RNA interaction assays
  • Protein-small molecule interaction assays
  • Analyte detection assays
  • Biomarker detection assays
  • Antibody detection assays
  • Enzymatic assays
  • Other immunoassays

In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym ""Alpha"" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

Specifications

Antibody Conjugates
Streptavidin
Application
Protein-protein interactions
Protein Analysis & Detection
Automation Compatible
Yes
Bead Type or Material
Alpha Donor
Brand
AlphaLISA
Species
Hamster
Unit Size
50 mg

Video gallery

Resources

1-3 of 3 Resources
Application Note Icon Image
Application Note
A Fast and Simple Chemiluminescent Assay for Monitoring of DNA-Protein Interactions

This note describes development of an AlphaLISA immunoassay to monitor the presence of specific DNA-binding proteins in nuclear...

Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...

Application Note Icon Image
Application Note
Biochemical binding ADCC assays utilizing AlphaLISA toolbox reagents for the characterization of hIgGs and FcγR1A.

Fc Gamma Receptors (FcγRs) are members of the immunoglobulin superfamily and play a critical role in the function of therapeutic...

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