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The Alpha SureFire® Ultra™ Multiplex phospho-STAT3 (Tyr705) + Total STAT3 assay kit is used to measure both the phosphorylation (on Tyr705) and total levels of endogenous STAT3 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure STAT3 phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
The Alpha SureFire Ultra Multiplex p-STAT3 + Total STAT3 assay kit is used to measure both phosphorylated and total levels of endogenous STAT3 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated STAT3 analysis, and the 545 nm (Tb) signal corresponds to the total STAT3 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.
In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.
Protein Analysis & Detection
|Assay Target Class
AlphaLISA SureFire Ultra Multiplex
500 Assay Points