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AlphaLISA SureFire Ultra Multiplex Human and Mouse Phospho-PDGFR-β (PDGF Receptor Beta) (Tyr751 & Total) Detection Kit, Control Lysate

Positive control lysate for Alpha SureFire Ultra Multiplex p-PDGF Receptor β (Tyr751) (Eu) + Total PDGF Receptor β (Tb) kit

Feature Specification
Application Protein Quantification

Positive control lysate for Alpha SureFire Ultra Multiplex p-PDGF Receptor β (Tyr751) (Eu) + Total PDGF Receptor β (Tb) kit

Product variant
Unit Size: 1 vial
Part #:
MPSU-PTPDGF-K-L
List price
USD 31.33
Your online price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

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Overview

This positive control lysate is prepared from NIH/3T3 cells, cultured to 80-90% confluence in T175 flasks in 10% FBS containing medium, medium removed and then treated with 50 ng/mL PDGF-BB for 5 minutes and lysed in 5 mL of 1X SureFire Ultra Lysis buffer.

Specifications

Application
Protein Quantification
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra Multiplex
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Phosphorylation
Product Group
Control Lysate
Shipping Conditions
Shipped in Blue Ice
Target Class
Phosphoproteins
Target Species
Human
Mouse
Technology
Alpha
Therapeutic Area
Metabolic
Oncology
Unit Size
1 vial

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Application Note
Characterizing chemokine receptor inhibitors with AlphaLISA SureFire Ultra, Alpha SureFire Ultra Multiplex and LANCE Ultra cAMP assays

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...

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