Positive control lysate for AlphaLISA™ SureFire® Ultra™ Multiplex Phospho/Total Insulin Receptor β Detection Kit.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Protein Quantification |
Positive control lysate for AlphaLISA™ SureFire® Ultra™ Multiplex Phospho/Total Insulin Receptor β Detection Kit.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
The positive control lysate is prepared from CHO cells transfected with the insulin receptor (CHO-INSR cells), cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 2μg/mL Insulin and 0.1% BSA for 15 minutes and lysed in 4mL of 1X SureFire Ultra Lysis buffer.
Application |
Protein Quantification
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra Multiplex
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
Insulin Receptor B
|
Target Class |
Phosphoproteins
|
Target Species |
Human
|
Technology |
Alpha
|
Unit Size |
1 Vial
|
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