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AlphaLISA SureFire Ultra Multiplex Human & Mouse Phospho-ERK1/2 (Thr202/Tyr204 & Total) Detection Kit, 500 Assay Points

The AlphaLISA™ SureFire® Ultra™ Multiplex phospho-ERK1/2 (Thr202/Tyr204) + Total ERK assay kit is used to measure both the phosphorylation of ERK1/2 and total levels of endogenous ERK1/2 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure ERK1/2phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Product Variants
Part number: MPSU-PTERK-M500
Unit Size: 500 Assay Points
List price: USD 4,223.00
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USD 4,223.00
USD 4,223.00 /each
Part number: MPSU-PTERK-M10K
Unit Size: 10,000 Assay Points
List price: USD 25,500.00
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USD 0.00
USD 25,500.00 /each
Part number: MPSU-PTERK-M50K
Unit Size: 50,000 Assay Points
List price: USD 80,040.00
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USD 0.00
USD 80,040.00 /each
Part number: MPSU-PTERK-M-HV
Unit Size: 100 Assay Points
List price: USD 1,250.00
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USD 0.00
USD 1,250.00 /each

Overview

The Alpha SureFire® Ultra™ Multiplex p-ERK1/2 (Thr202/Tyr204) (Eu) + Total ERK (Tb)assay kit is used to measure both phosphorylated and total levels of endogenous ERK1/2 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated ERK1/2 analysis, and the 545 nm (Tb) signal corresponds to the total ERK1/2 nalysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

Specifications

Application
Cell Signaling
Assay Target
ERK1/2
Assay Target Class
Phosphoproteins
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra Multiplex
Detection Method
Alpha
Molecular Modification
Phosphorylation
Product Group
Kit
Sample Volume
10 µL
Species
Human
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Cardiovascular
Central Nervous System
Metabolic
Neuroinflammation
Unit Size
500 Assay Points

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