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Multiplex Surefire Ultra Phospho-BTK (Tyr223 & Total) Detection Kit, 500 Assay Points

The Alpha SureFire® Ultra™ Multiplex phospho-BTK (Tyr223) + Total BTK assay kit is used to measure both the phosphorylation (on Tyr223) and total levels of endogenous BTK in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure BTK phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: MPSU-PTBTK-K500
Unit Size: 500 Assay Points
List price: USD 4,223.00
Your price:
USD 4,223.00
USD 4,223.00 /each
Part number: MPSU-PTBTK-K10K
Unit Size: 10,000 Assay Points
List price: USD 25,500.00
Your price:
USD 0.00
USD 25,500.00 /each
Part number: MPSU-PTBTK-K50K
Unit Size: 50,000 Assay Points
List price: USD 80,040.00
Your price:
USD 0.00
USD 80,040.00 /each
Part number: MPSU-PTBTK-K-HV
Unit Size: 100 Assay Points
List price: USD 1,250.00
Your price:
USD 0.00
USD 1,250.00 /each


The Alpha SureFire Ultra Multiplex p-BTK + Total BTX assay kit is used to measure both phosphorylated and total levels of endogenous BTK in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated BTX analysis, and the 545 nm (Tb) signal corresponds to the total BTXanalysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.


Protein Analysis & Detection
Assay Points
Assay Target Class
Automation Compatible
AlphaLISA SureFire Ultra Multiplex
Detection Method
Unit Size
500 Assay Points

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