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Alpha SureFire Ultra Lysis Buffer C (5X) - 10 mL

Additional Buffer C for select AlphaLISA® SureFire® Ultra assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: ALSU-LBC-10ML
Unit Size: 10 mL
List price: USD 165.74
Your price:
USD 165.74
USD 165.74 /each
Part number: ALSU-LBC-100ML
Unit Size: 100 mL
List price: USD 876.96
Your price:
USD 0.00
USD 876.96 /each

Overview

Lysis buffer C is already provided in the corresponding AlphaLISA SureFire Ultra kits, used in the detection of phosphoproteins in cell lysates. It is being offered as a standalone item, in case of need of replacement or for additional volume. It is intended to be used solely in conjunction with AlphaLISA SureFire Ultra assays.

Alpha SureFire Ultra Lysis Buffer C contains generic phosphatase inhibitors but no protease inhibitors. While we usually do not find protease inhibitors necessary, adding such protease inhibitors at standard concentrations will not interfere with the assay. The Alpha SureFire Ultra Lysis Buffer C formulation has been optimized to lyse the cells enough for making accessible the targets to be detected, while not releasing genomic DNA, in order to avoid a too viscous lysate solution that could potentially interfere with the pipetting process. The Alpha SureFire Ultra Lysis Buffer C has been developed to be a generally good buffer for all cell types and targets. Other lysis buffers may be tested, if necessary, but assay performance cannot be guaranteed under such conditions.

Specifications

Application
Protein Detection
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Method
Alpha
Experimental Type
In vitro
Shipping Conditions
Shipped in Blue Ice
Unit Size
10 mL

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