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NEXTFLEX Rapid DNA-Seq Kit 2.0

The NEXTFLEX® Rapid DNA-seq 2.0 kit is designed to prepare multiplexed libraries for single or paired-end sequencing using Illumina® platforms from fragmented DNA. This kit does not include enzymatic fragmentation.

For research use only. Not for use in diagnostic procedures.
Part Number: NOVA-5188-01
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Part Number: NOVA-5188-02
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Part Number: NOVA-5188-03
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    • Complete library prep solution, including size selection beads
    • Up to 1,536 UDI barcodes are available separately
    • Convenient and cost-effective package in an 8, 48, or 96 reaction format
    • Simplified workflow
    • Robust genome coverage
    • Reliable performance with GC-bias and sequencing bias mitigation
    • Low input requirement for genomic DNA down to 1 ng
    • Functionally tested with Illumina® sequencing platform
    • Automation compatible with liquid handlers, including Revvity’s Sciclone® G3 NGSx workstations, Zephyr® G3 NGS workstation, and BioQule NGS System
  • The NEXTFLEX  Rapid DNA-seq kit 2.0 produces library preps with a larger number of unique sequencing reads, allowing for robust genome coverage in challenging GC-rich sequences that can introduce bias.

    nextflex rapid DNA seq kit 2.0 fig-1

    Figure 1: Libraries prepared with NEXTFLEX  rapid DNA-Seq kit 2.0 show higher yield than libraries prepared with Competitor K’s kit using the same number of PCR cycles from a 250 ng and 1 ng input (A) and robust conversion rate (B).

    Additional Data between NEXTFLEX Rapid DNA-Seq 2.0 and Competitor K’s Kit Libraries
    nextflex rapid DNA seq kit 2.0 fig-2

    Figure 2: Libraries prepared with NEXTFLEX  Rapid DNA-seq 2.0 shows less GC bias than libraries prepared with Competitor K’s kit.

    nextflex rapid DNA seq kit 2.0 fig-3

    Figure 3: Libraries prepared with NEXTFLEX  Rapid DNA-seq 2.0 shows higher genome coverage than libraries prepared with Competitor K’s kit.

    Flexible Multiplexing Options

    The NEXTFLEX adapters are long, annealed adapters containing indexed sequences, which offer an improved multiplexing workflow and flexible experimental setups catered to individual needs. Our barcode portfolio encompasses options for single or paired-end multiplex sequencing. The NEXTFLEX Rapid DNA-seq kit 2.0 reagents are compatible with the NEXTFLEX Unique Dual Index Barcodes for up to 1,536 sample multiplexing.

    Automation Compatibility

    The NEXTFLEX Rapid DNA-seq Kit 2.0 is compatible with multiple liquid handlers, including the Revvity Sciclone G3 NGSx and Zephyr G3 NGS workstations.

    For low throughput automation, the NEXTFLEX Rapid DNA-seq Kit 2.0 kit is also now automated on the BioQule™ NGS System which simplifies both library prep AND quantitation. This low-cost, benchtop instrument delivers libraries ready to load into your sequencer with only 15 minutes of hands-on time.

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    Brand
    NEXTFLEX
    Type
    DNA-seq

Resources

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Application Note
NEXTFLEX Rapid 2.0 DNA-Seq App Note
whole genome sequencing library preparation, WGS library preparation, DNA-seq library prep, DNA sequencing, NEXTFLEX Rapid DNA-seq kit 2.0