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The NEXTFLEX® Rapid DNA-seq 2.0 kit is designed to prepare multiplexed libraries for single or paired-end sequencing using Illumina® platforms from fragmented DNA. This kit does not include enzymatic fragmentation.
The NEXTFLEX Rapid DNA-seq kit 2.0 produces library preps with a larger number of unique sequencing reads, allowing for robust genome coverage in challenging GC-rich sequences that can introduce bias.
Figure 1: Libraries prepared with NEXTFLEX rapid DNA-Seq kit 2.0 show higher yield than libraries prepared with Competitor K’s kit using the same number of PCR cycles from a 250 ng and 1 ng input (A) and robust conversion rate (B).
Figure 2: Libraries prepared with NEXTFLEX Rapid DNA-seq 2.0 shows less GC bias than libraries prepared with Competitor K’s kit.
Figure 3: Libraries prepared with NEXTFLEX Rapid DNA-seq 2.0 shows higher genome coverage than libraries prepared with Competitor K’s kit.
The NEXTFLEX adapters are long, annealed adapters containing indexed sequences, which offer an improved multiplexing workflow and flexible experimental setups catered to individual needs. Our barcode portfolio encompasses options for single or paired-end multiplex sequencing. The NEXTFLEX Rapid DNA-seq kit 2.0 reagents are compatible with the NEXTFLEX Unique Dual Index Barcodes for up to 1,536 sample multiplexing.
The NEXTFLEX Rapid DNA-seq Kit 2.0 is compatible with multiple liquid handlers, including the Revvity Sciclone G3 NGSx and Zephyr G3 NGS workstations.
For low throughput automation, the NEXTFLEX Rapid DNA-seq Kit 2.0 kit is also now automated on the BioQule™ NGS System which simplifies both library prep AND quantitation. This low-cost, benchtop instrument delivers libraries ready to load into your sequencer with only 15 minutes of hands-on time.
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