• UDI Barcodes for multiplexing RNA-seq libraries
• For multiplexing RNA-seq libraries compatible with both paired-end and single-read sequencing
• Every lot is tested for index purity and functionality by sequencing
• NEXTFLEX^® Rapid Directional RNA-Seq Kit 2.0 and the CRISPRclean^® Plus Stranded Total RNA Prep with rRNA Depletion

The NEXTFLEX^® RNA-seq 2.0 UDI barcodes (8-nt index) are conveniently plated at 6.25 µM stock concentration to be used with the NEXTFLEX^® Rapid Directional RNA-Seq kit 2.0 and the CRISPRclean^® Plus Stranded Total RNA Prep with rRNA Depletion.

Improve RNA-seq Data Integrity

The NEXTFLEX^® Unique Dual Index barcodes contain two unique indexes on each adapter, with a hamming distance of at least three throughout the entire series, to allow error correction. The NEXTFLEX^® Unique Dual Index RNA-seq barcodes can be used for PCR-free RNA sequencing applications. Patterned flow cells found on the Illumina^® NovaSeq^®, HiSeq^® 3000/4000, and HiSeq^® X platforms suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. Every lot is tested for index purity and functionality by sequencing.